Anti-Galectin-3/LGALS3 Polyclonal Antibody is a Rabbit antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat,Dog)

1. Sample:
SW480 (Human) Cell Lysate at 40 μg
Primary: Anti-Galectin 3 (TMAB-00735) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
2. Sample:
MCF-7 Cell (Human) Lysate at 40 μg
NIH/3T3 Cell (Mouse) Lysate at 40 μg
Primary: Anti-Galectin 3 (TMAB-00735) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Galectin 3 antibody (TMAB-00735)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Sample:
Lane 1: Siha (Human) Cell Lysate at 30 μg
Lane 2: U87MG (Human) Cell Lysate at 30 μg
Lane 3: A431 (Human) Cell Lysate at 30 μg
Lane 4: LOVO (Human) Cell Lysate at 30 μg
Lane 5: MCF-7 (Human) Cell Lysate at 30 μg
Lane 6: Hela (Human) Cell Lysate at 30 μg
Primary:
Anti-Galectin 3 (TMAB-00735) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
5. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (TMAB-00735) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Galectin 3

Human

LGALS3

Galectin-3

Nuclear Proteins,Cell differentiation

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.