Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Galectin-3/LGALS3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00735
Anti-Galectin-3/LGALS3 Polyclonal Antibody is a Rabbit antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Galectin-3/LGALS3 Polyclonal Antibody
Anti-Galectin-3/LGALS3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00735
Anti-Galectin-3/LGALS3 Polyclonal Antibody is a Rabbit antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse (predicted:Rat,Dog) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog) |
| Verified Activity | 1. Sample: SW480 (Human) Cell Lysate at 40 μg Primary: Anti-Galectin 3 (TMAB-00735) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa 2. Sample: MCF-7 Cell (Human) Lysate at 40 μg NIH/3T3 Cell (Mouse) Lysate at 40 μg Primary: Anti-Galectin 3 (TMAB-00735) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa 3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Galectin 3 antibody (TMAB-00735) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 4. Sample: Lane 1: Siha (Human) Cell Lysate at 30 μg Lane 2: U87MG (Human) Cell Lysate at 30 μg Lane 3: A431 (Human) Cell Lysate at 30 μg Lane 4: LOVO (Human) Cell Lysate at 30 μg Lane 5: MCF-7 (Human) Cell Lysate at 30 μg Lane 6: Hela (Human) Cell Lysate at 30 μg Primary: Anti-Galectin 3 (TMAB-00735) at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 28 kDa 5. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (TMAB-00735) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of thegalectinfamily of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014] |
| Immunogen | KLH conjugated synthetic peptide: human Galectin 3 |
| Antigen Species | Human |
| Gene ID | 3958 |
| Uniprot ID | |
| Biology Area | Nuclear Proteins,Cell differentiation |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-Galectin-3/LGALS3 Polyclonal Antibody | purchase Anti-Galectin-3/LGALS3 Polyclonal Antibody | Anti-Galectin-3/LGALS3 Polyclonal Antibody cost | order Anti-Galectin-3/LGALS3 Polyclonal Antibody
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.