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Anti-FGFR2 Polyclonal Antibody is a Rabbit antibody targeting FGFR2. Anti-FGFR2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-FGFR2 Polyclonal Antibody is a Rabbit antibody targeting FGFR2. Anti-FGFR2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat) |
Verified Activity | 1. Image was kindly submitted by Dr. Uthaman from Yale University. L6 cells were transfected with FGFR2, and stained with RABBIT ANTI-FGFR2 POLYCLONAL ANTIBODY, conjugated (TMAB-00675-FITC) at 1:100 dilution 2. Paraformaldehyde-fixed, paraffin embedded (human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Tissue/cell: MCF7; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 6. Sample: Lane 1: Mouse Spinal cord tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Human 293T cell lysates Lane 4: Human A431 cell lysates Lane 5: Human HeLa cell lysates Primary: Anti-FGFR2 (TMAB-00675) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 89 kDa Observed band size: 142 kDa |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle. Note=Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded.Isoform 3: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide: human FGFR2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | FGF,Receptor tyrosine kinases,FGF Receptors,Ectoderm,Neurogenesis,FGF,Receptor Tyrosine Kinases,Ectoderm |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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