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Anti-FGFR2 Polyclonal Antibody

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Catalog No. TMAB-00675

Anti-FGFR2 Polyclonal Antibody is a Rabbit antibody targeting FGFR2. Anti-FGFR2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-FGFR2 Polyclonal Antibody

Anti-FGFR2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00675
Anti-FGFR2 Polyclonal Antibody is a Rabbit antibody targeting FGFR2. Anti-FGFR2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-FGFR2 Polyclonal Antibody is a Rabbit antibody targeting FGFR2. Anti-FGFR2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Image was kindly submitted by Dr. Uthaman from Yale University. L6 cells were transfected with FGFR2, and stained with RABBIT ANTI-FGFR2 POLYCLONAL ANTIBODY, conjugated (TMAB-00675-FITC) at 1:100 dilution
2. Paraformaldehyde-fixed, paraffin embedded (human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Tissue/cell: MCF7; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (TMAB-00675) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
6. Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human 293T cell lysates
Lane 4: Human A431 cell lysates
Lane 5: Human HeLa cell lysates
Primary: Anti-FGFR2 (TMAB-00675) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 142 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle. Note=Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded.Isoform 3: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FGFR2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
FGF,Receptor tyrosine kinases,FGF Receptors,Ectoderm,Neurogenesis,FGF,Receptor Tyrosine Kinases,Ectoderm
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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