Antibody Type: Rabbit Polyclonal

Application: WB,FCM

Reactivity: Human,Mouse (predicted:Rat,Chicken,Cow,Rabbit,Sheep)

IgG

Human,Mouse (predicted:Rat,Chicken,Cow,Rabbit,Sheep)

1. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-bFGF (TMAB-00669) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
2. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-bFGF antibody (TMAB-00669)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-bFGF antibody (TMAB-00669)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human bFGF

Human

2247

FGF,Angiogenic growth factors,FGF,Kidney development,Neurogenesis,FGF,Extracellular