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Anti-ERG Antibody (2Q359) is a Rabbit antibody targeting ERG. Anti-ERG Antibody (2Q359) can be used in WB,ICC/IF,IHC,ChIP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-ERG Antibody (2Q359) is a Rabbit antibody targeting ERG. Anti-ERG Antibody (2Q359) can be used in WB,ICC/IF,IHC,ChIP. |
Synonyms | Transforming protein ERG, Transcriptional regulator ERG, ERG |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of ERG on Jurkat cell lysates using anti-ERG antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERG antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERG antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ERG antibody. Counter stained with hematoxylin. 5. ICC staining ERG in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining ERG in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1?C3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Erg genes encode for multiple proteins due to alternative splicing and alternative usage of initiation codons. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 55 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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