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Anti-EphB4 Antibody-PE (6A752)
🥰Excellent
Catalog No. TMAY-00504P
Anti-EphB4 Antibody-PE (6A752) is a PE-conjugated Mouse antibody targeting EphB4. Anti-EphB4 Antibody-PE (6A752) can be used in FCM.
Anti-EphB4 Antibody-PE (6A752)
Anti-EphB4 Antibody-PE (6A752)
🥰Excellent
Catalog No. TMAY-00504P
Anti-EphB4 Antibody-PE (6A752) is a PE-conjugated Mouse antibody targeting EphB4. Anti-EphB4 Antibody-PE (6A752) can be used in FCM.
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Product Introduction
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Chemical Properties
| Description | Anti-EphB4 Antibody-PE (6A752) is a PE-conjugated Mouse antibody targeting EphB4. Anti-EphB4 Antibody-PE (6A752) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 6A752 |
| Reactivity | Human |
| Verified Activity | 1. Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry. 2. Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (PE-conjugated, 10 µg/ml) at 37°C 1 hour. Positive staining was localized to plasma membrane. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 (rh EphB4; TMPY-00787; NP_004435.3; Met 1-Ala 539) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
| Conjucates | PE |
| Others Formats | Unconjugated/APC/FITC |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Human EphB4 protein (TMPY-00787) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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