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Anti-EphA2 Polyclonal Antibody
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Catalog No. TMAB-00624
Alias Sek-2, Sek2, Myk2, EPH receptor A2, Eck, AW545284
Anti-EphA2 Polyclonal Antibody is a Rabbit antibody targeting EphA2. Anti-EphA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-EphA2 Polyclonal Antibody
Anti-EphA2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00624Alias Sek-2, Sek2, Myk2, EPH receptor A2, Eck, AW545284
Anti-EphA2 Polyclonal Antibody is a Rabbit antibody targeting EphA2. Anti-EphA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-EphA2 Polyclonal Antibody is a Rabbit antibody targeting EphA2. Anti-EphA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Sek-2, Sek2, Myk2, EPH receptor A2, Eck, AW545284 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Monkey) |
| Verified Activity | 1. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-EphA2 (TMAB-00624) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kDa Observed band size: 115 kDa 2. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EphA2/EpHreceptor A2 Polyclonal Antibody, Unconjugated (TMAB-00624) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Human colon carcinoma lysates Lysate at 30 μg Intestine (Mouse) Lysate at 30 μg Primary: Anti-EphA2/EpHreceptor A2 (TMAB-00624) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/3000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa, 52/55 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Note=Present at regions of cell-cell contacts but also at the leading edge of migrating cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]. |
| Immunogen | KLH conjugated synthetic peptide: human EphA2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Eph Receptors,Growth Cone,Axonal Guidance Proteins,Kinases,Tyrosine Kinase Receptors |
| Molecular Weight | Theoretical: 105 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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