Anti-EphA2 Polyclonal Antibody is a Rabbit antibody targeting EphA2. Anti-EphA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Monkey)

1. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-EphA2 (TMAB-00624) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size: 115 kDa
2. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EphA2/EpHreceptor A2 Polyclonal Antibody, Unconjugated (TMAB-00624) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Human colon carcinoma lysates Lysate at 30 μg
Intestine (Mouse) Lysate at 30 μg
Primary: Anti-EphA2/EpHreceptor A2 (TMAB-00624) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/3000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa, 52/55 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human EphA2

Human

Eph Receptors,Growth Cone,Axonal Guidance Proteins,Kinases,Tyrosine Kinase Receptors