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Anti-EP300 Polyclonal Antibody
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Catalog No. TMAB-00620
Alias Protein propionyltransferase p300, Protein lactyltransferas p300, Protein 2-hydroxyisobutyryltransferase p300, p300 HAT, P300, Histone crotonyltransferase p300, Histone butyryltransferase p300, Histone acetyltransferase p300, Ep300, E1A-associated protein p300
Anti-EP300 Polyclonal Antibody is a Rabbit antibody targeting EP300. Anti-EP300 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-EP300 Polyclonal Antibody
Anti-EP300 Polyclonal Antibody
😃Good
Catalog No. TMAB-00620Alias Protein propionyltransferase p300, Protein lactyltransferas p300, Protein 2-hydroxyisobutyryltransferase p300, p300 HAT, P300, Histone crotonyltransferase p300, Histone butyryltransferase p300, Histone acetyltransferase p300, Ep300, E1A-associated protein p300
Anti-EP300 Polyclonal Antibody is a Rabbit antibody targeting EP300. Anti-EP300 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-EP300 Polyclonal Antibody is a Rabbit antibody targeting EP300. Anti-EP300 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Protein propionyltransferase p300, Protein lactyltransferas p300, Protein 2-hydroxyisobutyryltransferase p300, p300 HAT, P300, Histone crotonyltransferase p300, Histone butyryltransferase p300, Histone acetyltransferase p300, Ep300, E1A-associated protein p300 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-Ep300 (TMAB-00620) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 264 kDa Observed band size: 264 kDa 2. Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-Ep300 antibody (TMAB-00620) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Ep300 (TMAB-00620) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 264 kDa Observed band size: 264 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EP300) Polyclonal Antibody, Unconjugated (TMAB-00620) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Co-localizes with ROCK2 in the nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human EP300 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Rb family,Bromodomains,ChIP antibodies,Acetylation,Other factors,Hypoxia,Obesity,Nuclear |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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