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Anti-DLAT Polyclonal Antibody

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Catalog No. TMAB-00550
Alias Pyruvate dehydrogenase complex component E2 (PDC-E2;PDCE2), mitochondrial, M2 antigen complex 70 kDa subunit, DLTA, DLAT, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC)

Anti-DLAT Polyclonal Antibody is a Rabbit antibody targeting DLAT. Anti-DLAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DLAT Polyclonal Antibody

Anti-DLAT Polyclonal Antibody

😃Good
Catalog No. TMAB-00550Alias Pyruvate dehydrogenase complex component E2 (PDC-E2;PDCE2), mitochondrial, M2 antigen complex 70 kDa subunit, DLTA, DLAT, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC)
Anti-DLAT Polyclonal Antibody is a Rabbit antibody targeting DLAT. Anti-DLAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-DLAT Polyclonal Antibody is a Rabbit antibody targeting DLAT. Anti-DLAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasPyruvate dehydrogenase complex component E2 (PDC-E2;PDCE2), mitochondrial, M2 antigen complex 70 kDa subunit, DLTA, DLAT, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC)
Ig Type
IgG
Reactivity
Human,Mouse,Rat,Zebrafish (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig,Cat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PDCE2) Polyclonal Antibody, Unconjugated (TMAB-00550) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Mouse Muscle tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Muscle tissue lysates
Lane 6: Human PANC-1 cell lysates
Primary: Anti-PDCE2 (TMAB-00550) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion matrix.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PDCE2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism of carbohydrates,Carbohydrate metabolism,Energy Metabolism,Mitochondrial markers,Cancer,Energy Metabolism,Mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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