Anti-CysLT1 Polyclonal Antibody is a Rabbit antibody targeting CysLT1. Anti-CysLT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Sheep)

1. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-CysLT1 (TMAB-04909) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 59 kD
2. Sample: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-CysLT1 (TMAB-04909) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 59 kD
3. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CysLT1 Polyclonal Antibody, Unconjugated (TMAB-04909) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CYSLTR1

Human

CYSLTR1

Cysteinyl leukotriene receptor 1

Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.