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Anti-CXCL1 Polyclonal Antibody

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Catalog No. TMAB-00495
Alias Secretory protein N51, Scyb1, Platelet-derived growth factor-inducible protein KC, Mgsa, Gro1, gro, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, α), chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

Anti-CXCL1 Polyclonal Antibody is a Rabbit antibody targeting CXCL1. Anti-CXCL1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CXCL1 Polyclonal Antibody

Anti-CXCL1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00495Alias Secretory protein N51, Scyb1, Platelet-derived growth factor-inducible protein KC, Mgsa, Gro1, gro, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, α), chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Anti-CXCL1 Polyclonal Antibody is a Rabbit antibody targeting CXCL1. Anti-CXCL1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-CXCL1 Polyclonal Antibody is a Rabbit antibody targeting CXCL1. Anti-CXCL1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasSecretory protein N51, Scyb1, Platelet-derived growth factor-inducible protein KC, Mgsa, Gro1, gro, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, α), chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GRO Alpha) Polyclonal Antibody, Unconjugated (TMAB-00495) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GRO Alpha) Polyclonal Antibody, Unconjugated (TMAB-00495) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GRO Alpha Polyclonal Antibody, Unconjugated (TMAB-00495) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93% and 82% identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse GRO Alpha
Antigen Species
Mouse
Gene ID
Uniprot ID
Biology Area
Alpha Chemokines (CXC)
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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