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Anti-CPT2 Antibody (3I417)

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Catalog No. TMAC-00953
Alias mitochondrial, CPT2, CPT1, Carnitine palmitoyltransferase II (CPT II), Carnitine O-palmitoyltransferase 2, mitochondrial

Anti-CPT2 Antibody (3I417) is a Rabbit antibody targeting CPT2. Anti-CPT2 Antibody (3I417) can be used in WB,ICC/IF,IHC.

Anti-CPT2 Antibody (3I417)

Anti-CPT2 Antibody (3I417)

😃Good
Catalog No. TMAC-00953Alias mitochondrial, CPT2, CPT1, Carnitine palmitoyltransferase II (CPT II), Carnitine O-palmitoyltransferase 2, mitochondrial
Anti-CPT2 Antibody (3I417) is a Rabbit antibody targeting CPT2. Anti-CPT2 Antibody (3I417) can be used in WB,ICC/IF,IHC.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4967-10 days
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Product Introduction

Bioactivity
Description
Anti-CPT2 Antibody (3I417) is a Rabbit antibody targeting CPT2. Anti-CPT2 Antibody (3I417) can be used in WB,ICC/IF,IHC.
Synonymsmitochondrial, CPT2, CPT1, Carnitine palmitoyltransferase II (CPT II), Carnitine O-palmitoyltransferase 2, mitochondrial
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of CPT2 on different lysates using anti-CPT2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293, Lane 3: HepG2, Lane 4: NIH/3T3.
2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CPT2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.
5. ICC staining CPT2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining CPT2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 74 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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