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Anti-CHKL Polyclonal Antibody

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Catalog No. TMAB-04306

Anti-CHKL Polyclonal Antibody is a Rabbit antibody targeting CHKL. Anti-CHKL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CHKL Polyclonal Antibody

Anti-CHKL Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-04306
Anti-CHKL Polyclonal Antibody is a Rabbit antibody targeting CHKL. Anti-CHKL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CHKL Polyclonal Antibody is a Rabbit antibody targeting CHKL. Anti-CHKL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Cow,Horse,Sheep)
Verified Activity
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CHKL Polyclonal Antibody, Unconjugated (TMAB-04306) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
IHC-P
IHC-Fr
IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCholine kinase (CK) and ethanolamine kinase (EK) catalyzethe phosphorylation of choline/ethanolamine tophosphocholine/phosphoethanolamine. This is the first enzyme in thebiosynthesis of phosphatidylcholine/phosphatidylethanolamine in allanimal cells. The highly purified CKs from mammalian sources andtheir recombinant gene products have been shown to have EK activityalso, indicating that both activities reside on the same protein.The choline kinase-like protein encoded by CHKL belongs to thecholine/ethanolamine kinase family; however, its exact function isnot known. Read-through transcripts are expressed from this locusthat include exons from the downstream CPT1B locus. [provided byRefSeq, Jun 2009].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CHKL
Antigen Species
Human
Gene Name
CHKB
Gene ID
1120
Protein Name
Choline/ethanolamine kinase
Uniprot ID
Q9Y259
Function
Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis.
Chemical Properties
Molecular WeightTheoretical: 45 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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