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Anti-CD73 Antibody-PE (9U110)
🥰Excellent
Catalog No. TMAY-01317P
Anti-CD73 Antibody-PE (9U110) is a PE-conjugated Mouse antibody targeting CD73. Anti-CD73 Antibody-PE (9U110) can be used in FCM.
Anti-CD73 Antibody-PE (9U110)
Anti-CD73 Antibody-PE (9U110)
🥰Excellent
Catalog No. TMAY-01317P
Anti-CD73 Antibody-PE (9U110) is a PE-conjugated Mouse antibody targeting CD73. Anti-CD73 Antibody-PE (9U110) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD73 Antibody-PE (9U110) is a PE-conjugated Mouse antibody targeting CD73. Anti-CD73 Antibody-PE (9U110) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 9U110 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human NT5E(CD73) expression on human whole blood lymphocytes. Cells were stained with PE-conjugated anti-Human NT5E(CD73). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; TMPY-01851; NP_002517.1; Met1-Lys547) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Research Background | 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium. |
| Conjucates | PE |
| Others Formats | Unconjugated/FITC |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human CD73 / NT5E Protein (TMPY-01851) |
| Antigen Species | Human |
| Biology Area | Phosphatases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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