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Anti-CD59a Antibody-PE (1M258)
🥰Excellent
Catalog No. TMAY-03434P
Anti-CD59a Antibody-PE (1M258) is a PE-conjugated Rabbit antibody targeting CD59a. Anti-CD59a Antibody-PE (1M258) can be used in FCM.
Anti-CD59a Antibody-PE (1M258)
Anti-CD59a Antibody-PE (1M258)
🥰Excellent
Catalog No. TMAY-03434P
Anti-CD59a Antibody-PE (1M258) is a PE-conjugated Rabbit antibody targeting CD59a. Anti-CD59a Antibody-PE (1M258) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD59a Antibody-PE (1M258) is a PE-conjugated Rabbit antibody targeting CD59a. Anti-CD59a Antibody-PE (1M258) can be used in FCM. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1M258 |
| Reactivity | Mouse |
| Verified Activity | Flow cytometric analysis of Mouse CD59a expression on BABL/c bone marrow erythroid cells. Cells were stained with PE-conjugated anti-Mouse CD59a. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of erythroid cells. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD59a (rM CD59a; TMPY-02266; NP_001104530.1; Met1-Lys95) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Research Background | Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins. It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria. |
| Conjucates | PE |
| Others Formats | Unconjugated/APC/FITC |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Mouse CD59a Protein (TMPY-02266) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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