Antibody Type: Rabbit Polyclonal

Application: FCM

Reactivity: Human (predicted:Rat,Mouse)

IgG

Human (predicted:Rat,Mouse)

1. Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CD45 antibody (TMAB-00374), Dilution: 1 μg in 100 1 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-CD45/PTPRC/FITC Conjugated antibody (TMAB-00374-FITC)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature.
3. Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-CD45 antibody (TMAB-00374)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-CD45 antibody (TMAB-00374)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Blank control: U937. Primary Antibody (green line): Rabbit Anti-CD45 antibody (TMAB-00374)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: rat CD45/B220

Rat

24699

Endothelial Cell Markers,CD,Regulatory T Cells,CD,Non-lineage,Microglia marker,Tyrosine Phosphatases,B Lymphocytic Lineage,NK Cell Lineage,T Lymphocytic Lineage,Dendritic Cell Lineage,Mast Cell Lineage,Monocytic Lineage,Neutrophil Lineage,Surface Molecules