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Anti-CD31/PECAM-1 Antibody-FITC (1O74) is a FITC-conjugated Rabbit antibody targeting CD31/PECAM-1. Anti-CD31/PECAM-1 Antibody-FITC (1O74) can be used in FCM.
Description | Anti-CD31/PECAM-1 Antibody-FITC (1O74) is a FITC-conjugated Rabbit antibody targeting CD31/PECAM-1. Anti-CD31/PECAM-1 Antibody-FITC (1O74) can be used in FCM. |
Ig Type | Monoclonal Rabbit IgG |
Clone | 1O74 |
Reactivity | Mouse |
Verified Activity | Flow cytometric analysis of Mouse PECAM1(CD31) expression on BABL/c splenocytes. Cells were stained with FITC-conjugated anti-Mouse PECAM1(CD31). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
Application | FCM |
Recommended Dose | 5 μl/Test, 0.1 mg/ml |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD31/PECAM-1 (rM CD31/PECAM-1; TMPY-01619; NP_033868.3; Met1-Lys590) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
Purification | Protein A |
Appearance | Liquid |
Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
Research Background | The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. |
Conjucates | FITC |
Others Formats | Unconjugated/APC/PE |
Antibody Types Available | 4 |
Immunogen | Recombinant Mouse CD31/PECAM-1 Protein (TMPY-01619) |
Antigen Species | Mouse |
Biology Area | Hemangioblast Markers, ITIM/ITAM Immunoreceptors and Related Molecules |
Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
Transport | Shipping with blue ice. |
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