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Anti-CD21 Antibody (2Z920) is a Rabbit antibody targeting CD21. Anti-CD21 Antibody (2Z920) can be used in WB,ICC,IHC,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-CD21 Antibody (2Z920) is a Rabbit antibody targeting CD21. Anti-CD21 Antibody (2Z920) can be used in WB,ICC,IHC,IP,FCM. |
Synonyms | SLEB9, EBV receptor, CVID7, CR2, CD21, C3DRSLEB9, C3DR |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Western blot analysis of CD21 on different lysates using anti-CD21 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi, Lane 2: Raji. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin. 5. ICC staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CR21 plays an important role in enhancing mature B cell responses to foreign antigens. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 130-155 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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