Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse)

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse)

1. Blank control (blue line): U251 (blue).
Primary Antibody (green line): Rabbit Anti-caspase-8 antibody (TMAB-00290)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (TMAB-00290) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Jurkat (Human) Cell Lysate at 30 μg
K562 (Human) Cell Lysate at 30 μg
U87Mg (Human) Cell Lysate at 30 μg
Primary: Anti-caspase-8 subunit p18 (TMAB-00290) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55/18 kDa
Observed band size: 57 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1μg /Test

Polyclonal

KLH conjugated synthetic peptide: rat Caspase-8 subunit p10

Rat

64044

Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis