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Anti-Aquaporin-9/AQP9 Polyclonal Antibody
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Catalog No. TMAB-00143
Anti-Aquaporin-9/AQP9 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-9/AQP9. Anti-Aquaporin-9/AQP9 Polyclonal Antibody can be used in FCM.
Anti-Aquaporin-9/AQP9 Polyclonal Antibody
Anti-Aquaporin-9/AQP9 Polyclonal Antibody
😃Good
Catalog No. TMAB-00143
Anti-Aquaporin-9/AQP9 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-9/AQP9. Anti-Aquaporin-9/AQP9 Polyclonal Antibody can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: FCM Reactivity: Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
| Verified Activity | Blank control: A549 (blue). Primary Antibody: Rabbit Anti-AQP9/AF647 Conjugated antibody (TMAB-00143/AF647), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange),used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min). The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (TMAB-00143/AF647, 1 μg/1x10^6 cells) for 30 min on ice. |
| Application | FCM |
| Recommended Dose | FCM: 1μg /test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. A new member of the AQP family, AQP9, has been cloned from human leukocytes. It is homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and in lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it does not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. |
| Immunogen | KLH conjugated synthetic peptide: human AQP9 |
| Antigen Species | Human |
| Gene ID | 366 |
| Uniprot ID | |
| Biology Area | Cancer,Channels |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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