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Anti-ANXA4 Antibody (3T392)
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Catalog No. TMAC-00214
Anti-ANXA4 Antibody (3T392) is a Rabbit antibody targeting ANXA4. Anti-ANXA4 Antibody (3T392) can be used in WB,ICC,IF,IHC,FCM.
Anti-ANXA4 Antibody (3T392)
Anti-ANXA4 Antibody (3T392)
😃Good
Catalog No. TMAC-00214
Anti-ANXA4 Antibody (3T392) is a Rabbit antibody targeting ANXA4. Anti-ANXA4 Antibody (3T392) can be used in WB,ICC,IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-ANXA4 Antibody (3T392) is a Rabbit antibody targeting ANXA4. Anti-ANXA4 Antibody (3T392) can be used in WB,ICC,IF,IHC,FCM. |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Annexin IV on human liver tissue lysate using anti-Annexin IV antibody at 1/2,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin IV antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin IV antibody. Counter stained with hematoxylin. 4. ICC staining Annexin IV in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Annexin IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Annexin IV in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of HepG2 cells with Annexin IV antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca++-dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino terminus with possible Ca++ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl- flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human Annexin IV aa 1-200 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
ProteinIIProtein IIPP4-XPlacental anticoagulant protein II (PAP-II)PAP-IIPAPIIP32.5P 32.5LipocortinIVLipocortin IVEndonexinIEndonexin IChromobindin-4Chromobindin4Carbohydrate-binding protein p33/p41ANXA4ANXA 4ANX4ANX 4AnnexinIVAnnexin-A4Annexin-4Annexin4Annexin IVAnnexin A435-β calcimedin35β calcimedin35-beta calcimedin35-b calcimedin
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