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Anti-AKAP12 Polyclonal Antibody
😃Good
Catalog No. TMAB-00084
Anti-AKAP12 Polyclonal Antibody is a Rabbit antibody targeting AKAP12. Anti-AKAP12 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AKAP12 Polyclonal Antibody
Anti-AKAP12 Polyclonal Antibody
😃Good
Catalog No. TMAB-00084
Anti-AKAP12 Polyclonal Antibody is a Rabbit antibody targeting AKAP12. Anti-AKAP12 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-AKAP12 Polyclonal Antibody is a Rabbit antibody targeting AKAP12. Anti-AKAP12 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining. 2. Sample: Lane1: mouse bladder Lysate at 25 μg Lane2: mouse lung Lysate at 25 μg Primary: Anti-AKAP12 (TMAB-00084) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 191 kDa Observed band size: 191 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human AKAP12 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Neurodegenerative disease,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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