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Anti-AK3L1 Antibody-PE (1J714)
🥰Excellent
Catalog No. TMAY-02328P
Anti-AK3L1 Antibody-PE (1J714) is a PE-conjugated Mouse antibody targeting AK3L1. Anti-AK3L1 Antibody-PE (1J714) can be used in FCM.
Anti-AK3L1 Antibody-PE (1J714)
Anti-AK3L1 Antibody-PE (1J714)
🥰Excellent
Catalog No. TMAY-02328P
Anti-AK3L1 Antibody-PE (1J714) is a PE-conjugated Mouse antibody targeting AK3L1. Anti-AK3L1 Antibody-PE (1J714) can be used in FCM.
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Chemical Properties
| Description | Anti-AK3L1 Antibody-PE (1J714) is a PE-conjugated Mouse antibody targeting AK3L1. Anti-AK3L1 Antibody-PE (1J714) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 1J714 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human AK4 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with PE Mouse anti-Human AK4. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AK4 / Adenylate Kinase 4 / AK3L1 (rh AK4 / Adenylate Kinase 4 / AK3L1; TMPY-04469; P27144-1; Ala2-Tyr223) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Research Background | Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously. |
| Conjucates | PE |
| Others Formats | Unconjugated |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Human AK4 / Adenylate Kinase 4 / AK3L1 protein (TMPY-04469) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. |
| Transport | Shipping with blue ice. |
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