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AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 (Synonyms: F(ab')₂ Fragment Goat...)
Catalog No. TMAB-02062AF488 Copy Product Info
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AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 is a AF488-conjugated Goat antibody targeting IgG (H+L). AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 can be used in FCM,ICC/IF,IF.
AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488
Copy Product Info🥰Excellent
Catalog No. TMAB-02062AF488
Synonyms F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (AF488), F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/BF488, F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (AF488), BF488 AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)
AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 is a AF488-conjugated Goat antibody targeting IgG (H+L). AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 can be used in FCM,ICC/IF,IF.
AffiniPure F
(ab')2 Fragment Goat Anti-Rabbit IgG
(H+L) Secondary Antibody-AF488
(ab')2 Fragment Goat Anti-Rabbit IgG
(H+L) Secondary Antibody-AF488
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 μL | $65 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 is a AF488-conjugated Goat antibody targeting IgG (H+L). AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) Secondary Antibody-AF488 can be used in FCM,ICC/IF,IF. |
| Synonyms | F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (AF488), F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/BF488, F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (AF488), BF488 AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) |
| Reactivity | Rabbit |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062AF488), DAPI (blue) was used to stain the cell nuclei.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062AF488), DAPI (blue) was used to stain the cell nuclei. 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062AF488), DAPI (blue) was used to stain the cell nuclei. 4. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062AF488), DAPI (blue) was used to stain the cell nuclei.     |
| Application | |
| Recommended Dose | IF: 1:200-1000; ICC/IF: 1:100-1000; FCM: 1:100-1000 |
| Antibody Type | Polyclonal |
| Host Species | Goat |
| Construction | Polyclonal Antibody |
| Research Background | Target: Rabbit Host: Goat Antibody Format: F(ab') Fragment Specificity: IgG (H+L) Minimal Cross Reactivity: Human, Mouse, Rat Serum Proteins Conjugate: Alexa Fluor 488 (Amax: 493 Emax: 519nm) Product Category: F(ab') Fragment Affinity-Purified Antibodies Dilution Range: 1:100-800 for most applications Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Information: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species. F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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