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VEGF165 Protein, Human, Recombinant (Verified Cell Activity)
(Synonyms: VEGFA, VEGF-165, VEGF165, Vascular Endothelial Growth Factor Isoform 165) Copy Product InfoSynonyms: VEGFA, VEGF-165, VEGF165, Vascular Endothelial Growth Factor Isoform 165
Catalog No. TMPR-00002 Copy Product Info
VEGF165 Protein, Human, Recombinant (Verified Cell Activity) is expressed in Baculovirus Insect Cells. The accession number is NP_001165097.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µg | $3,424 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.50-12.0 ng/mL. |
| Description | VEGF165 Protein, Human, Recombinant (Verified Cell Activity) is expressed in Baculovirus Insect Cells. The accession number is NP_001165097. |
| Expression System | Baculovirus Insect Cells |
| Tag | Tag Free |
| Accession Number | NP_001165097 |
| Construction | Ala27-Arg191, Disulfide-linked homodimer |
| Endotoxin | <0.01 EU per 1 μg of the protein by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100‑500 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin. |
| Synonyms | VEGFA, VEGF-165, VEGF165, Vascular Endothelial Growth Factor Isoform 165 |
| Research Background | Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling. |
| Molecular Weight | 20-22 kDa (reducing conditions); 39-42 kDa (non-reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: VEGF165 Protein, Human, Recombinant (Verified Cell Activity) chemical structure | VEGF165 Protein, Human, Recombinant (Verified Cell Activity) molecular weight
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