VEGF164 Protein, Mouse, Recombinant (Yeast)

Name: VEGF164 Protein, Mouse, Recombinant (Yeast)
Brand: TargetMol
SKU: TMPY-01717U
Price: 969 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Vpf, Vegf, vascular endothelial growth factor A) Copy Product Info

Synonyms: Vpf, Vegf, vascular endothelial growth factor A

Catalog No. TMPY-01717U Copy Product Info

VEGF164 Protein, Mouse, Recombinant (Yeast) is expressed in P. pastoris (Yeast). The accession number is Q00731-2.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$76	-	In Stock
10 µg	$123	-	In Stock
20 µg	$197	-	In Stock
50 µg	$372	-	In Stock
100 µg	$597	7-10 days	7-10 days
200 µg	$969	7-10 days	7-10 days
500 µg	Preferential	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:> 97% as analyzed by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 6.0 ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of > 1.7 × 10^5 units/mg.
Description	VEGF164 Protein, Mouse, Recombinant (Yeast) is expressed in P. pastoris (Yeast). The accession number is Q00731-2.
Species	Mouse
Expression System	P. pastoris (Yeast)
Tag	Tag Free
Accession Number	Q00731-2
Construction	Ala27-Arg190 Expressed with an N-terminal Met
Protein Purity	> 97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in 25 mM HEPES, 150 mM NaCl, pH 7.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Vpf, Vegf, vascular endothelial growth factor A
Research Background	Vascular Endothelial Growth Factor (VEGF) was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer of KDR/Flt in VEGF signal transduction remain to be elucidated. In vivo, VEGF has been found to be a potent angiogenesis inducer.

Chemical Properties

Molecular Weight

39 kDa (Non-reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.