VEGF121 Protein, Human, Recombinant (E. coli)

Name: VEGF121 Protein, Human, Recombinant (E. coli)
Brand: TargetMol
SKU: TMPS-00168
Rating: 4.5 (1 reviews)

(Synonyms: VPF, VEGF-121, Vascular Permeability Factor, Vascular Endothelial Growth Factor 121) Copy Product Info

Synonyms: VPF, VEGF-121, Vascular Permeability Factor, Vascular Endothelial Growth Factor 121

Catalog No. TMPS-00168 Copy Product Info

VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9.

Pack Size	Price	USA Stock	Global Stock
5 µg	$98	7-10 days	7-10 days
10 µg	$170	7-10 days	7-10 days
20 µg	$295	7-10 days	7-10 days
50 µg	$622	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 5.0 ng/ml, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2.0 × 10 5 units/mg.
Description	VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P15692-9
Construction	Pro28-Arg147
Protein Purity	> 95% as determined by SDS-PAGE; > 95% as determined by HPLC
Endotoxin	< 0.2 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	VPF, VEGF-121, Vascular Permeability Factor, Vascular Endothelial Growth Factor 121
Research Background	VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development.

Chemical Properties

Molecular Weight

~28.2 kDa (Non-reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.