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VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 μg | $170 | 7-10 days | |
50 μg | $622 | 7-10 days |
Biological Activity | ED 50 < 5.0 ng/ml, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2.0 × 10 5 units/mg. |
Description | VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9. |
Species | Human |
Expression System | E. coli |
Tag | Tag Free |
Accession Number | P15692-9 |
Synonyms | VPF,VEGF-121,Vascular Permeability Factor,Vascular Endothelial Growth Factor 121 |
Construction | Pro28-Arg147 |
Protein Purity | > 95% as determined by SDS-PAGE; > 95% as determined by HPLC |
Molecular Weight | ~28.2 kDa (Non-reducing conditions) |
Endotoxin | < 0.2 EU/μg of protein as determined by the LAL method. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development. |
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