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TNF alpha Protein, Porcine, Recombinant

Catalog No. TMPS-00138

TNF alpha Protein, Porcine, Recombinant is expressed in E. coli. The accession number is P23563.

TNF alpha Protein, Porcine, Recombinant

TNF alpha Protein, Porcine, Recombinant

Catalog No. TMPS-00138
TNF alpha Protein, Porcine, Recombinant is expressed in E. coli. The accession number is P23563.
Pack SizePriceAvailabilityQuantity
10 μg $887-10 days
50 μg $1967-10 days
1 mg $1,5307-10 days
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Product Information

Biological Activity
ED 50 < 20.0 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 5.0 × 10 4 units/mg.
Description
TNF alpha Protein, Porcine, Recombinant is expressed in E. coli. The accession number is P23563.
Species
Porcine
Expression System
E. coli
TagTag Free
Accession NumberP23563
Synonyms
Tumor necrosis factor ligand superfamily member 2,TNFSF2,TNF-alpha,TNF-a,TNFA,DIF,Cachectin
Construction
Arg78Leu232
Protein Purity
> 98% as determined by SDS-PAGE; > 98% as determined by HPLC
Molecular Weight~17.2 kDa (Reducing conditions)
Endotoxin< 0.2 EU/μg of protein as determined by the LAL method.
FormulationLyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune disease including Crohn's disease, rheumatoid arthritis and graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

Dose Conversion

You can also refer to dose conversion for different animals. More

Sci Citations

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.