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SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi)
Catalog No. TMPY-05727 Copy Product Info
3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi) is expressed in E. coli expression system. The predicted molecular weight is 36.57 kDa and the accession number is YP_009725295.1.
SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi)
Catalog No. TMPY-05727
Copy Product Info3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi) is expressed in E. coli expression system. The predicted molecular weight is 36.57 kDa and the accession number is YP_009725295.1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $77 | 7-10 days | 7-10 days | |
| 10 μg | $123 | 7-10 days | 7-10 days | |
| 20 μg | $198 | 7-10 days | 7-10 days | |
| 50 μg | $397 | 7-10 days | 7-10 days | |
| 100 μg | $793 | 7-10 days | 7-10 days |
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | 3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi) is expressed in E. coli expression system. The predicted molecular weight is 36.57 kDa and the accession number is YP_009725295.1. |
| Species | SARS-CoV-2 |
| Expression System | E. coli |
| Tag | N-His-Avi |
| Accession Number | YP_009725295.1 |
| Construction | A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) 3CL Protease (YP_009725295.1) (Ser3264-Gln3569) was expressed with a N-terminal polyhistidine tag followed by a MBP tag. Predicted N terminal: Met |
| Protein Purity | > 95 % as determined by SDS-PAGE. |
| Molecular Weight | 36.57 kDa (predicted) |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Research Background | 3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. |
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