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PHOSPHO1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30.67 kDa and the accession number is Q8TCT1-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $812 | 7-10 days |
Biological Activity | Activity testing is not tested. It is theoretically active, but we cannot guarantee it. |
Description | PHOSPHO1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30.67 kDa and the accession number is Q8TCT1-1. |
Species | Human |
Expression System | E. coli |
Tag | N-His |
Accession Number | Q8TCT1-1 |
Synonyms | phosphatase, orphan 1 |
Construction | Met1-Cys267 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 30.67 kDa (Predicted and reducing conditions) |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS, 300mM L-Arginine (pH 7.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | PHOSPHO1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralisation. |
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