MIF Protein, Human, Recombinant (His)

Name: MIF Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02095
Price: 109 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: MMIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), GLIF, GIF) Copy Product Info

Synonyms: MMIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), GLIF, GIF

Catalog No. TMPY-02095 Copy Product Info

MIF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13.8 kDa and the accession number is P14174.

Pack Size	Price	USA Stock	Global Stock
5 µg	$109	-	In Stock
10 µg	$182	-	In Stock
20 µg	$296	-	In Stock
50 µg	$600	-	In Stock
100 µg	$923	7-10 days	7-10 days
200 µg	$1,420	7-10 days	7-10 days
500 µg	$2,520	7-10 days	7-10 days
1 mg	$3,910	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:94.9%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	MIF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13.8 kDa and the accession number is P14174.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P14174
Construction	A DNA sequence encoding the human MIF (P14174) (Pro 2-Ala 115) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Predicted N terminal: Pro 2
Protein Purity	> 92 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	MMIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), GLIF, GIF
Research Background	Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is a ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of the central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread, and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important role in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.

Chemical Properties

Molecular Weight

13.8 kDa (predicted); 19 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.