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MERS-CoV Nucleocapsid Protein (His)

(Synonyms: Nucleo, NP Protein, MERS-CoV, novel coronavirus Nucleoprotein Protein, MERS-CoV, ncov NP Protein, MERS-CoV, cov np Protein, MERS-CoV, coronavirus Nucleoprotein Protein, MERS-CoV, coronavirus Nucleocapsid Protein, MERS-CoV, coronavirus NP Protein, MERS-CoV) Copy Product Info

Synonyms: Nucleo, NP Protein, MERS-CoV, novel coronavirus Nucleoprotein Protein, MERS-CoV, ncov NP Protein, MERS-CoV, cov np Protein, MERS-CoV, coronavirus Nucleoprotein Protein, MERS-CoV, coronavirus Nucleocapsid Protein, MERS-CoV, coronavirus NP Protein, MERS-CoV

Catalog No. TMPY-03423 Copy Product Info
MERS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.51 kDa and the accession number is K0BVN3.
MERS-CoV Nucleocapsid Protein (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$68-In Stock
10 µg$108-In Stock
20 µg$177-In Stock
50 µg$347-In Stock
100 µg$583-In Stock
200 µg$9937-10 days7-10 days
500 µg$2,0107-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:94%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
MERS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.51 kDa and the accession number is K0BVN3.
Species
MERS-CoV
Expression System
Baculovirus Insect Cells
TagC-His
Accession NumberK0BVN3
ConstructionA DNA sequence encoding the MERS-CoV Nucleoprotein (AFS88943.1) (Met1-Asp413) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Met 1
Protein Purity
> 90 % as determined by SDS-PAGE
MERS-CoV Nucleocapsid Protein (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsNucleo, NP Protein, MERS-CoV, novel coronavirus Nucleoprotein Protein, MERS-CoV, ncov NP Protein, MERS-CoV, cov np Protein, MERS-CoV, coronavirus Nucleoprotein Protein, MERS-CoV, coronavirus Nucleocapsid Protein, MERS-CoV, coronavirus NP Protein, MERS-CoV
Research Background
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Chemical Properties
Molecular Weight46.51 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: MERS-CoV Nucleocapsid Protein (His) chemical structure | MERS-CoV Nucleocapsid Protein (His) molecular weight