LIF Protein, Mouse, Recombinant

Name: LIF Protein, Mouse, Recombinant
Brand: TargetMol
SKU: TMPY-04278
Rating: 4.5 (1 reviews)

(Synonyms: leukemia inhibitory factor) Copy Product Info

Synonyms: leukemia inhibitory factor

Catalog No. TMPY-04278 Copy Product Info

Purity: 96.2%

LIF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.9 kDa and the accession number is P09056.

Pack Size	Price	USA Stock	Global Stock
5 µg	$43	7-10 days	7-10 days
10 µg	$66	7-10 days	7-10 days
20 µg	$106	In Stock	In Stock
50 µg	$252	7-10 days	7-10 days
100 µg	$498	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:96.2%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
Description	LIF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.9 kDa and the accession number is P09056.
Species	Mouse
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P09056
Construction	A DNA sequence encoding the mouse LIF (NP_032527.1) (Met1-Phe203) was expressed and purified. Predicted N terminal: Ser 24
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	leukemia inhibitory factor
Research Background	Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.

Chemical Properties

Molecular Weight

19.9 kDa (predicted); 34.1 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.