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LIF Protein, Mouse, Recombinant (E. coli)
(Synonyms: LIF, Differentiation-stimulating factor, D factor) Copy Product InfoSynonyms: LIF, Differentiation-stimulating factor, D factor
Catalog No. TMPX-00003 Copy Product Info
LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | Inquiry | Inquiry | Inquiry | |
| 100 μg | Inquiry | Inquiry | Inquiry | |
| 500 μg | Inquiry | Inquiry | Inquiry |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures. |
| Description | LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056. |
| Species | Mouse |
| Expression System | E. coli |
| Tag | Tag Free |
| Accession Number | P09056 |
| Amino Acid | SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF |
| Construction | 24-203 aa |
| Protein Purity | > 98% as determined by SDS-PAGE and HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02% Tween 20. |
| Reconstitution | Reconstitute the lyophilized protein in sterile deionized water containing 0.1% BSA. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | LIF, Differentiation-stimulating factor, D factor |
| Molecular Weight | 19.9 kDa (Predicted) |
| Relative Density. | no data available |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: LIF Protein, Mouse, Recombinant (E. coli) chemical structure | LIF Protein, Mouse, Recombinant (E. coli) molecular weight
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