LIF Protein, Human, Recombinant (E. coli)

Name: LIF Protein, Human, Recombinant (E. coli)
Brand: TargetMol
SKU: TMPS-00147
Price: 98 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: MLPLI, Melanoma-derived LPL inhibitor, Leukemia inhibitory factor, HILDA, Emfilermin, Differentiation- stimulating factor, DIA, D-FACTOR, D factor, CDF) Copy Product Info

Synonyms: MLPLI, Melanoma-derived LPL inhibitor, Leukemia inhibitory factor, HILDA, Emfilermin, Differentiation- stimulating factor, DIA, D-FACTOR, D factor, CDF

Catalog No. TMPS-00147 Copy Product Info

LIF Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15018.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$98	-	In Stock
10 µg	$170	-	In Stock
20 µg	$268	7-10 days	7-10 days
50 µg	$497	7-10 days	7-10 days
100 µg	$772	7-10 days	7-10 days
200 µg	$1,180	7-10 days	7-10 days
500 µg	$2,160	7-10 days	7-10 days
1 mg	$3,390	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95% as determined by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 0.2 ng/ml, measured by a cell differentiation assay using TF-I cells, corresponding to a specific activity of > 5.0 × 10 6 units/mg.
Description	LIF Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15018.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P15018
Construction	Ser23-Phe202
Protein Purity	> 95% as determined by SDS-PAGE
Endotoxin	< 0.2 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	MLPLI, Melanoma-derived LPL inhibitor, Leukemia inhibitory factor, HILDA, Emfilermin, Differentiation- stimulating factor, DIA, D-FACTOR, D factor, CDF
Research Background	Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.

Chemical Properties

Molecular Weight

~19.7 kDa (Reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.