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JBP1 Protein, Leishmania infantum, Recombinant (His & V5)

(Synonyms: Thymine dioxygenase JBP1, Thymidine hydroxylase JBP1, JBP1, J-binding protein 1) Copy Product Info

Synonyms: Thymine dioxygenase JBP1, Thymidine hydroxylase JBP1, JBP1, J-binding protein 1

Catalog No. TMPH-02403 Copy Product Info
JBP1 Protein, Leishmania infantum, Recombinant (His & V5) is expressed in E. coli expression system with N-10xHis-V5 tag. The predicted molecular weight is 53.4 kDa and the accession number is A4HU70.
JBP1 Protein, Leishmania infantum, Recombinant (His & V5)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$12920 days20 days
10 µg$21620 days20 days
20 µg$36020 days20 days
50 µg$54320 days20 days
100 µg$74520 days20 days
200 µg$1,07020 days20 days
500 µg$1,73020 days20 days
1 mg$2,53020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
JBP1 Protein, Leishmania infantum, Recombinant (His & V5) is expressed in E. coli expression system with N-10xHis-V5 tag. The predicted molecular weight is 53.4 kDa and the accession number is A4HU70.
Species
Leishmania infantum
Expression System
E. coli
TagN-10xHis-V5
Accession NumberA4HU70
Amino AcidSCIACLDGQFKGLALAFDDFGINVLMQPRDVMIFDSHHFHSNTEVELSFSGEDWKRLTCVFYYRAALGEPASYAEYRRRLEKSKQDTSFTPVVSNVRVKENGTNLNRPSPVYPIFLSPFWVPMVAHCLQHCASEAQCVHDAMTADGSRLAEVMFGEPLSTSDGIPLRGEEEKLKANSDSASRPLSRLGGFSETNLMVSTAVEKKKYLNSEFLSHFISAQLLDMWKQARGKWLELVGREWTHMLALNPERKDFLWRNQSEMNSAFFDLCEVGKQVMLGLLGKEAALPKEEQAFWTMYAVHLNAACAEELNMPHVAMSLRKLNVKLKDFNFGGTRYFKDMPPEEQKRRMERKQRIEEARRHGMSSGAHEKRANWLTNDSFDYQTEDCVFDYAQHKWVPPALHA
Construction200-600 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsThymine dioxygenase JBP1, Thymidine hydroxylase JBP1, JBP1, J-binding protein 1
Research Background
Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions.
Chemical Properties
Molecular Weight53.4 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: JBP1 Protein, Leishmania infantum, Recombinant (His & V5) chemical structure | JBP1 Protein, Leishmania infantum, Recombinant (His & V5) molecular weight