**HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated**

Name: HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated
Brand: TargetMol
SKU: TMPK-01802
Rating: 4.5 (1 reviews)

(Synonyms: TRP53, TP53, P53, MHC, LFS1, HLA-A, FLJ92943, BCC7, Antigen NY-CO-13) Copy Product Info

Synonyms: TRP53, TP53, P53, MHC, LFS1, HLA-A, FLJ92943, BCC7, Antigen NY-CO-13

Catalog No. TMPK-01802 Copy Product Info

HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.5 ng/ml determined by ELISA.
Description	HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A140T913(HLA-A02:01)&P61769(B2M)&HMTEVVRHC.
Species	Human
Expression System	E. coli
Tag	C-His-Avi
Accession Number	A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC
Construction	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin	< 1 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	TRP53, TP53, P53, MHC, LFS1, HLA-A, FLJ92943, BCC7, Antigen NY-CO-13
Research Background

Chemical Properties

Molecular Weight

35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated chemical structure | HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated molecular weight