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HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated
(Synonyms: NLVPMVATV, CMVpp65) Copy Product InfoSynonyms: NLVPMVATV, CMVpp65
Catalog No. TMPK-01807 Copy Product Info
HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $87 | 7-10 days | 7-10 days | |
| 10 µg | $133 | 7-10 days | 7-10 days | |
| 20 µg | $198 | 7-10 days | 7-10 days | |
| 50 µg | $377 | 7-10 days | 7-10 days | |
| 100 µg | $598 | 7-10 days | 7-10 days | |
| 200 µg | $948 | 7-10 days | 7-10 days | |
| 500 µg | $1,760 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1 ng/ml determined by ELISA. |
| Description | HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV. |
| Species | Human |
| Expression System | E. coli |
| Tag | C-His-Avi |
| Accession Number | A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV |
| Construction | Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | NLVPMVATV, CMVpp65 |
| Research Background | Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population. |
| Molecular Weight | 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated chemical structure | HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated molecular weight
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