SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)

Catalog No. TMPJ-01429

The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)

Catalog No. TMPJ-01429

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$112	7-10 days	7-10 days
10 μg	$183	7-10 days	7-10 days
20 μg	$295	7-10 days	7-10 days
50 μg	$558	7-10 days	7-10 days
100 μg	$919	7-10 days	7-10 days
200 μg	$1,520	7-10 days	7-10 days
500 μg	$2,970	7-10 days	7-10 days
1 mg	$4,480	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.
Species	SARS-CoV-2
Expression System	E. coli
Tag	N-6xHis
Accession Number	YP_009725309.1
Synonyms	SARS-CoV 2 nsp14,SARS-CoV 2 ExoN,Guanine-N7 methyltransferase
Amino Acid	Ala1-Gln527
Construction	Ala1-Gln527
Molecular Weight	60 KDa (reducing condition)
Formulation	Supplied as a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 2 mM DTT, 20% Glycerol, 0.1% TritonX-100, pH 6.0.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Shipping with blue ice.
Research Background	The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

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