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SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)

Catalog No. TMPJ-01429
Synonyms: SARS-CoV 2 nsp14, Guanine-N7 methyltransferase, SARS-CoV 2 ExoN

The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

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SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 184.00
50 μg 5 days $ 558.00
500 μg 5 days $ 2,970.00
1 mg 5 days $ 4,480.00
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Biological Description
Technical Params
Product Properties
Description The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.
Species SARS-CoV-2
Expression System E. coli
Tag N-6His
Accession Number YP_009725309.1
Synonyms SARS-CoV 2 nsp14, Guanine-N7 methyltransferase, SARS-CoV 2 ExoN
Amino Acid Ala1-Gln527
Construction Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.
Molecular Weight 60 KDa, reducing conditions
Formulation Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 2mM DTT, 20% Glycerol, 0.1% TritonX-100, pH 6.0.
Stability & Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Shipping

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Research Background The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

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Keywords

SARS-CoV-2 Guanine-N7 methyltransferase Protein (His) SARS-CoV 2 nsp14 Guanine-N7 methyltransferase SARS-CoV 2 ExoN recombinant recombinant-proteins proteins protein

 

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