GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His)

Catalog No. TMPY-02451

GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.7 kDa and the accession number is AAB65663.

GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His)

Catalog No. TMPY-02451

GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.7 kDa and the accession number is AAB65663.

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100 μg	$255	In Stock
200 μg	$436	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity Assay
Description	GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.7 kDa and the accession number is AAB65663.
Species	Aequorea victoria
Expression System	E. coli
Tag	N-His
Accession Number	AAB65663
Synonyms	GFP Protein
Construction	A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser2-Lys238) was expressed with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Molecular Weight	28.7 kDa (predicted); 34 kDa (reducing conditions)
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.