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Synonyms: GFP Protein

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $35 | - | In Stock | |
| 10 µg | $52 | - | In Stock | |
| 20 µg | $81 | - | In Stock | |
| 50 µg | $153 | 7-10 days | 7-10 days | |
| 100 µg | $255 | 7-10 days | 7-10 days | |
| 200 µg | $436 | 7-10 days | 7-10 days |
| Bioactivity | Activity Assay |
| Description | GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.7 kDa and the accession number is AAB65663. |
| Species | Aequorea victoria |
| Expression System | E. coli |
| Tag | N-His |
| Accession Number | AAB65663 |
| Construction | A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser2-Lys238) was expressed with a polyhistide tag at the N-terminus. Predicted N terminal: Met |
| Protein Purity | ≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC. ,![]() |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | GFP Protein |
| Research Background | The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity. |
| Molecular Weight | 28.7 kDa (predicted); 34 kDa (reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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