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Gellan lyase Protein, Geobacillus stearothermophilus, Recombinant (His)

(Synonyms: Gellan lyase) Copy Product Info

Synonyms: Gellan lyase

Catalog No. TMPH-00767 Copy Product Info
Cleaves the glycosidic bonds of gellan backbone and releases tetrasaccharide units of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the non-reducing terminal. The enzyme is highly specific to the heteropolysaccharide gellan. Gellan lyase Protein, Geobacillus stearothermophilus, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 24.5 kDa and the accession number is P85513.
Gellan lyase Protein, Geobacillus stearothermophilus, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 μg$129-In Stock
10 μg$21620 days20 days
20 μg$360-In Stock
50 μg$54320 days20 days
100 μg$74520 days20 days
200 μg$1,07020 days20 days
500 μg$1,73020 days20 days
1 mg$2,53020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:92%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Description
Cleaves the glycosidic bonds of gellan backbone and releases tetrasaccharide units of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the non-reducing terminal. The enzyme is highly specific to the heteropolysaccharide gellan. Gellan lyase Protein, Geobacillus stearothermophilus, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 24.5 kDa and the accession number is P85513.
Species
Geobacillus stearothermophilus
Expression System
E. coli
TagN-6xHis
Accession NumberP85513
Amino AcidLVSESNPGRAIPAGGKGATIRAARPGLATTLNGPKAGNGTTGATKLTTPARPLSEGANMMCDHRAGGNAAISGSSVGEGTARAGDSKVMSRMLSPKGSIIAGTVNMMPADIAAGSVRTPSSLPPDGRSATPMSVSEVASDISHKDGSVNVTKDPVTAAGLTAMRKNANKGSPPASPLPLKADNKGVHINKHWVDLKNDNDFNTR
Construction1-204 aa
Protein Purity
> 90% as determined by SDS-PAGE.
https://cdn.targetmol.com/group3/M00/3F/D9/CgoaEGh2NMaEVyuWAAAAALgdiJ8529.jpg
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationTris-based buffer, 50% glycerol
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsGellan lyase
Research Background
Chemical Properties
Molecular Weight24.5 kDa (predicted)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Gellan lyase Protein, Geobacillus stearothermophilus, Recombinant (His) chemical structure | Gellan lyase Protein, Geobacillus stearothermophilus, Recombinant (His) molecular weight