GAD2/GAD65 Protein, Human, Recombinant (GST)

Name: GAD2/GAD65 Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPY-04699
Price: 61 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), GAD65) Copy Product Info

Synonyms: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), GAD65

Catalog No. TMPY-04699 Copy Product Info

GAD65 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 92.6 kDa and the accession number is Q05329.

GAD2/GAD65 Protein, Human, Recombinant (GST)

Pack Size	Price	USA Stock	Global Stock
5 µg	$61	-	In Stock
10 µg	$97	-	In Stock
20 µg	$158	7-10 days	7-10 days
50 µg	$297	7-10 days	7-10 days
100 µg	$517	-	In Stock
200 µg	$883	7-10 days	7-10 days
500 µg	$1,780	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:91.1%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	GAD65 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 92.6 kDa and the accession number is Q05329.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	N-GST
Accession Number	Q05329
Construction	A DNA sequence encoding the human GAD2 (NP_000809.1) (Met1-Leu585) was expressed with a GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM GSH, pH8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), GAD65
Research Background	Glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of thegroup II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

92.6 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.