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FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His & Avi), Biotinylated
(Synonyms: FcRn alpha chain, FCRN, FCGRT&B2M, FCGRT) Copy Product InfoSynonyms: FcRn alpha chain, FCRN, FCGRT&B2M, FCGRT
Catalog No. TMPK-01684 Copy Product Info
FcRn Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P13599(FCGRT)&P07151(B2M).
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Biotinylated Rat FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200) (QC Test). |
| Description | FcRn Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P13599(FCGRT)&P07151(B2M). |
| Species | Rat |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P13599 (FCGRT) & P07151 (B2M) |
| Construction | Ala23-Ser298 (FCGRT) and Ile21-Met119 (B2M) |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | FcRn alpha chain, FCRN, FCGRT&B2M, FCGRT |
| Research Background |
| Molecular Weight | 33.9 kDa (FCGRT) and 11.63 kDa (B2M) (Predicted); 45-60 kDa (FCGRT) and 12-14 kDa (B2M) (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His & Avi), Biotinylated chemical structure | FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His & Avi), Biotinylated molecular weight
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