ERCC3 Protein, Human, Recombinant (GST)

Name: ERCC3 Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPH-01380
Rating: 4.5 (1 reviews)

(Synonyms: XPBC, XPB, Xeroderma pigmentosum group B-complementing protein, TFIIH subunit XPB, TFIIH basal transcription factor complex 89 kDa subunit (TFIIH 89 kDa subunit;TFIIH p89), General transcription and DNA repair factor IIH helicase/translocase subunit XPB, ERCC3, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, Basic transcription factor 2 89 kDa subunit (BTF2 p89)) Copy Product Info

Synonyms: XPBC, XPB, Xeroderma pigmentosum group B-complementing protein, TFIIH subunit XPB, TFIIH basal transcription factor complex 89 kDa subunit (TFIIH 89 kDa subunit;TFIIH p89), General transcription and DNA repair factor IIH helicase/translocase subunit XPB, ERCC3, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, Basic transcription factor 2 89 kDa subunit (BTF2 p89)

Catalog No. TMPH-01380 Copy Product Info

ERCC3 Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 115.6 kDa and the accession number is P19447.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$129	20 days	20 days
10 µg	$216	20 days	20 days
20 µg	$360	20 days	20 days
50 µg	$543	20 days	20 days
100 µg	$745	20 days	20 days
200 µg	$1,070	20 days	20 days
500 µg	$1,730	20 days	20 days
1 mg	$2,530	20 days	20 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	ERCC3 Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 115.6 kDa and the accession number is P19447.
Species	Human
Expression System	E. coli
Tag	N-GST
Accession Number	P19447
Amino Acid	MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK
Construction	1-782 aa
Protein Purity	> 85% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	XPBC, XPB, Xeroderma pigmentosum group B-complementing protein, TFIIH subunit XPB, TFIIH basal transcription factor complex 89 kDa subunit (TFIIH 89 kDa subunit;TFIIH p89), General transcription and DNA repair factor IIH helicase/translocase subunit XPB, ERCC3, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, Basic transcription factor 2 89 kDa subunit (BTF2 p89)
Research Background	ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Chemical Properties

Molecular Weight

115.6 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.