ENTPD5 Protein, Mouse, Recombinant (His)

Name: ENTPD5 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-04276
Rating: 4.5 (1 reviews)

(Synonyms: Pcph, NTPDase-5, NTPDase5, mNTPase, ER-UDPase, ectonucleoside triphosphate diphosphohydrolase 5, Cd39l4, AI987697, AI196558) Copy Product Info

Synonyms: Pcph, NTPDase-5, NTPDase5, mNTPase, ER-UDPase, ectonucleoside triphosphate diphosphohydrolase 5, Cd39l4, AI987697, AI196558

Catalog No. TMPY-04276 Copy Product Info

ENTPD5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.6 kDa and the accession number is Q9WUZ9.

Pack Size	Price	USA Stock	Global Stock
5 µg	$108	7-10 days	7-10 days
10 µg	$178	7-10 days	7-10 days
20 µg	$296	7-10 days	7-10 days
50 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to hydrolyze the 5’phosphate groups from the substrate guanosine 5’diphosphate (GDP). The specific activity is > 7000 pmols/min/μg.
Description	ENTPD5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.6 kDa and the accession number is Q9WUZ9.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q9WUZ9
Construction	A DNA sequence encoding the mouse ENTPD5 (Q9WUZ9) (Thr19-Ser427) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Thr 19
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	Pcph, NTPDase-5, NTPDase5, mNTPase, ER-UDPase, ectonucleoside triphosphate diphosphohydrolase 5, Cd39l4, AI987697, AI196558
Research Background	Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

Chemical Properties

Molecular Weight

46.6 kDa (predicted); 45 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.