ENPP2 Protein, Human, Recombinant (His)

Name: ENPP2 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02778
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: PDNP2, PD-Iα, PD-IALPHA, NPP2, LysoPLD, ectonucleotide pyrophosphatase/phosphodiesterase 2, AUTOTAXIN, ATX-X, ATX) Copy Product Info

Synonyms: PDNP2, PD-Iα, PD-IALPHA, NPP2, LysoPLD, ectonucleotide pyrophosphatase/phosphodiesterase 2, AUTOTAXIN, ATX-X, ATX

Catalog No. TMPY-02778 Copy Product Info

ENPP2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 96 kDa and the accession number is AAH34961.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$52	-	In Stock
10 µg	$81	-	In Stock
20 µg	$129	7-10 days	7-10 days
50 µg	$255	-	In Stock
100 µg	$451	7-10 days	7-10 days
200 µg	$797	7-10 days	7-10 days
500 µg	$1,690	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 96.9%; SEC-HPLC: 98.9%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	ENPP2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 96 kDa and the accession number is AAH34961.1.
Species	Human
Expression System	HEK293 Cells
Tag	N-His
Accession Number	AAH34961.1
Construction	A DNA sequence encoding the mature form of human ENPP2 (AAH34961.1) (Asp 49-Ile 863) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 88 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	PDNP2, PD-Iα, PD-IALPHA, NPP2, LysoPLD, ectonucleotide pyrophosphatase/phosphodiesterase 2, AUTOTAXIN, ATX-X, ATX
Research Background	ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

Chemical Properties

Molecular Weight

96 kDa (predicted); 120-130 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.