Endo H Protein, Streptomyces plicatus, Recombinant (His)

Catalog No. TMPH-00514

Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.

Endo H Protein, Streptomyces plicatus, Recombinant (His)

Catalog No. TMPH-00514

Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.

Pack Size	Price	Availability
5 μg	$143	20 days
10 μg	$238	20 days
20 μg	$397	20 days
50 μg	$597	20 days
100 μg	$845	20 days

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User Guide of Recombinant Proteins

Product Information

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.
Species	Streptomyces plicatus
Expression System	P. pastoris (Yeast)
Tag	C-6xHis
Accession Number	P04067
Synonyms	Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase H,Endoglycosidase H (Endo H),Endo-beta-N-acetylglucosaminidase H,DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase H
Amino Acid	APVKQGPTSVAYVEVNNNSMLNVGKYTLADGGGNAFDVAVIFAANINYDTGTKTAYLHFNENVQRVLDNAVTQIRPLQQQGIKVLLSVLGNHQGAGFANFPSQQAASAFAKQLSDAVAKYGLDGVDFDDEYAEYGNNGTAQPNDSSFVHLVTALRANMPDKIISLYNIGPAASRLSYGGVDVSDKFDYAWNPYYGTWQVPGIALPKAQLSPAAVEIGRTSRSTVADLARRTVDEGYGVYLTYNLDGGDRTADVSAFTRELYGSEAVRTP
Construction	45-313 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Molecular Weight	31.2 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.