DUSP3 Protein, Human, Recombinant

Name: DUSP3 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-03482
Rating: 4.5 (1 reviews)

(Synonyms: VHR, dual specificity phosphatase 3) Copy Product Info

Synonyms: VHR, dual specificity phosphatase 3

Catalog No. TMPY-03482 Copy Product Info

DUSP3 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 20.6 kDa and the accession number is P51452.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by hydrolysis of 250 µM 3-O-methyl fluorescein phosphate (OMFP). The specific activity is >1.0 µmol/min/mg.
Description	DUSP3 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 20.6 kDa and the accession number is P51452.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	P51452
Construction	A DNA sequence encoding the human VHR (P51452)(Met 1-Pro 185)was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	VHR, dual specificity phosphatase 3
Research Background	Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP), and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs, VHR lacks mitogen-activated protein kinase (MAPK)-binding domain, and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates, it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and, therefore, may play a role in aspects of T lymphocyte physiology that depend on these kinases.

Chemical Properties

Molecular Weight

20.6 kDa (predicted); 21 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.