CDC25B Protein, Human, Recombinant (GST)

Catalog No. TMPC-00103 Copy Product Info

CDC25B Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P30305.

CDC25B Protein, Human, Recombinant (GST)

Catalog No. TMPC-00103

Copy Product Info

CDC25B Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P30305.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 μg	$1,280	35 days	35 days

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	The specific activity of CDC25B was determined to be 21 nmol phosphate released /min/mg by a phosphatase assay.
Description	CDC25B Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P30305.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	P30305
Synonyms	M-phase inducer phosphatase 2,CDC25HU2,CDC25B
Construction	Recombinant full length human CDC25B was expressed in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>70% as determined by SDS-PAGE.
Molecular Weight	~110 kDa
Formulation	Supplied as sterile 20 mM MOPS, pH 7.5, 300 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	CDC25 phosphatases are key cell cycle regulators and represent very attractive but challenging targets for anticancer drug discovery. CDC25B may be used as a potential prognostic marker in the pathogenesis of retinoblastoma. In mitosis, Kiz is a new substrate of CDC25B whose dephosphorylation following CDC25B stabilization leads to the formation of multipolar spindles. Furthermore, endogenous Kiz and CDC25B interact only in mitosis, suggesting that Kiz phosphorylation depends on a balance between CDC25B and Plk1 activities. The abnormal expression of CDC25B phosphatases detected in a number of tumors implies that their dysregulation is involved in malignant transformation. Both CDC25B and PED/PEA-15 play a certain role in the carcinogenesis of esophageal cancer, they will be the new diagnostic/therapeutic targets in esophageal carcinoma.