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CD122/IL2RB Protein, Rat, Recombinant (His & Avi), Biotinylated
(Synonyms: IL2RBC, Il2rb) Copy Product InfoSynonyms: IL2RBC, Il2rb
Catalog No. TMPY-07251 Copy Product Info
CD122/IL2RB Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P26896.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $208 | 7-10 days | 7-10 days | |
| 10 µg | $343 | 7-10 days | 7-10 days | |
| 20 µg | $588 | 7-10 days | 7-10 days | |
| 50 µg | $939 | 7-10 days | 7-10 days | |
| 100 µg | $1,600 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Loaded Recombinant Rat IL-2R beta/CD122 Protein, His Tag, Biotinylated on SA Biosensor, can bind Recombinant Rat IL-2 Protein with an affinity constant of 6.27 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). |
| Description | CD122/IL2RB Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P26896. |
| Species | Rat |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P26896 |
| Construction | A DNA sequence encoding the Rat IL-2R beta / CD122 (NP_037327.1) (Met1-Glu239) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Protein Purity | ≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Synonyms | IL2RBC, Il2rb |
| Research Background | Interleukin-2 receptor (IL-2R) also known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms concerning the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high-affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high-affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development. |
| Molecular Weight | 28.17 kDa (predicted); 41 kDa (reducing contition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: CD122/IL2RB Protein, Rat, Recombinant (His & Avi), Biotinylated chemical structure | CD122/IL2RB Protein, Rat, Recombinant (His & Avi), Biotinylated molecular weight
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