Complement C5 Protein, Human, Recombinant (Flag)

Name: Complement C5 Protein, Human, Recombinant (Flag)
Brand: TargetMol
SKU: TMPY-06814
Rating: 4.5 (1 reviews)

(Synonyms: ECLZB, CPAMD4, complement component 5, Complement C5, C5D, C5b, C5a) Copy Product Info

Synonyms: ECLZB, CPAMD4, complement component 5, Complement C5, C5D, C5b, C5a

Catalog No. TMPY-06814 Copy Product Info

Complement C5 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with Flag tag. The predicted molecular weight is 187.36 kDa and the accession number is NP_001726.2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days
200 µg	$1,230	7-10 days	7-10 days
500 µg	$2,530	7-10 days	7-10 days
1 mg	$5,280	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Complement C5 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with Flag tag. The predicted molecular weight is 187.36 kDa and the accession number is NP_001726.2.
Species	Human
Expression System	HEK293 Cells
Tag	N-Flag
Accession Number	NP_001726.2
Construction	A DNA sequence encoding the human C5 (NP_001726.2) (Gln19-Cys1676) was expressed with a flag tag at the N-terminus. Predicted N terminal: Asp
Protein Purity	≥ 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM MOPS, 150 mM NaCl, 0.05% CHAPS, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	ECLZB, CPAMD4, complement component 5, Complement C5, C5D, C5b, C5a
Research Background	C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.

Chemical Properties

Molecular Weight

187.36 kDa (predicted); 176.6 kDa, 100.87 kDa and 72.07 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.